Changes in Transforming Growth
Factor-ß1 in Gingival Crevicular Fluid of Patients with Chronic Periodontitis Following Periodontal Flap Surgery
Published: February 1, 2015 | DOI: https://doi.org/10.7860/JCDR/2015/.5539
Vignesh Vikram, Ramakrishnan T., Anilkumar K., Ambalavanan N.
1. PG Student, Department of Periodontology, Meenakshi Ammal Dental College and Hospital, Maduravoyal, Chennai, India.
2. Professor, Department of Periodontology, Meenakshi Ammal Dental College and Hospital, Maduravoyal, Chennai, India.
3. Reader, Department of Periodontology, Meenakshi Ammal Dental College and Hospital, Maduravoyal, Chennai, India.
4. Professor & HOD, Department of Periodontology, Meenakshi Ammal Dental College and Hospital, Maduravoyal, Chennai, India.
Correspondence
Dr. Vignesh Vikram,
PG Student, Department of Periodontology, Meenakshi Ammal Dental College and Hospital, Maduravoyal, Chennai-95, India.
E-mail: vigneshvikram87@gmail.com
Introduction: The aim of the study was to assess changes in Transforming Growth Factor– ß 1 (TGF- ß 1) levels in gingival crevicular fluid (GCF) and examine correlation of TGF- ß 1 levels and periodontal parameters: probing pocket depth (PPD) and clinical attachment level (CAL) in sites with chronic periodontitis at various time intervals before and after periodontal surgery.
Materials and Methods: The effects of non-surgical and surgical therapy on periodontal parameters and GCF TGF- ß 1 levels in 18 sites affected with chronic periodontitis were assessed upto 6 weeks post conventional flap surgery. GCF was collected and PPD, CAL recorded at Baseline. GCF was collected and PPD, CAL recorded at Pre-surgery and flap surgery performed. GCF was collected from all sites at Two Weeks Post-surgery. GCF was collected and PPD, CAL recorded at Six Weeks Post-surgery. The concentration of TGF- ß 1 in GCF was determined using a human TGF- ß 1 enzyme immunometric assay kit.
Results: Mean TGF- ß 1 concentrations were significantly reduced at Pre-surgery, at Two Weeks Post-surgery and at Six Weeks Post-surgery (p<0.05) when compared to Baseline TGF- ß 1 values. There was a statistically significant reduction in PPD and gain in CAL following non-surgical and surgical therapy when compared to baseline (p<0.05).
Conclusion: Results indicate that TGF- ß 1 may play a role in the pathogenesis and diagnosis of periodontal disease and could be considered as a disease predictive biomarker.
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